Plasmid
Superplasm

Part:BBa_K076013:Design

Designed by: iGEM2008 Princeton team   Group: iGEM08_Princeton   (2008-10-29)

pLV-TRE-Lbx1-T2A-GLRA1-P2A-D5R-Ubc-Bla


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 328
    Illegal EcoRI site found at 1452
    Illegal EcoRI site found at 3304
    Illegal XbaI site found at 2875
    Illegal XbaI site found at 3298
    Illegal SpeI site found at 2802
    Illegal SpeI site found at 8621
    Illegal SpeI site found at 9295
    Illegal PstI site found at 3921
    Illegal PstI site found at 11828
    Illegal PstI site found at 12615
    Illegal PstI site found at 13053
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 328
    Illegal EcoRI site found at 1452
    Illegal EcoRI site found at 3304
    Illegal NheI site found at 1572
    Illegal SpeI site found at 2802
    Illegal SpeI site found at 8621
    Illegal SpeI site found at 9295
    Illegal PstI site found at 3921
    Illegal PstI site found at 11828
    Illegal PstI site found at 12615
    Illegal PstI site found at 13053
    Illegal NotI site found at 865
    Illegal NotI site found at 9331
    Illegal NotI site found at 10053
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 328
    Illegal EcoRI site found at 1452
    Illegal EcoRI site found at 3304
    Illegal BglII site found at 626
    Illegal BglII site found at 4085
    Illegal BglII site found at 4151
    Illegal BglII site found at 4192
    Illegal BglII site found at 8384
    Illegal BglII site found at 9383
    Illegal BglII site found at 12181
    Illegal BamHI site found at 1201
    Illegal XhoI site found at 2863
    Illegal XhoI site found at 9338
    Illegal XhoI site found at 12936
    Illegal XhoI site found at 14047
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 328
    Illegal EcoRI site found at 1452
    Illegal EcoRI site found at 3304
    Illegal XbaI site found at 2875
    Illegal XbaI site found at 3298
    Illegal SpeI site found at 2802
    Illegal SpeI site found at 8621
    Illegal SpeI site found at 9295
    Illegal PstI site found at 3921
    Illegal PstI site found at 11828
    Illegal PstI site found at 12615
    Illegal PstI site found at 13053
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 328
    Illegal EcoRI site found at 1452
    Illegal EcoRI site found at 3304
    Illegal XbaI site found at 2875
    Illegal XbaI site found at 3298
    Illegal SpeI site found at 2802
    Illegal SpeI site found at 8621
    Illegal SpeI site found at 9295
    Illegal PstI site found at 3921
    Illegal PstI site found at 11828
    Illegal PstI site found at 12615
    Illegal PstI site found at 13053
    Illegal NgoMIV site found at 843
    Illegal NgoMIV site found at 1095
    Illegal NgoMIV site found at 4780
    Illegal NgoMIV site found at 5813
    Illegal NgoMIV site found at 5874
    Illegal NgoMIV site found at 5988
    Illegal NgoMIV site found at 11856
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4174
    Illegal BsaI site found at 9365
    Illegal BsaI.rc site found at 2114
    Illegal BsaI.rc site found at 3934
    Illegal BsaI.rc site found at 7516
    Illegal BsaI.rc site found at 9254
    Illegal BsaI.rc site found at 11736
    Illegal BsaI.rc site found at 11877
    Illegal BsaI.rc site found at 13191
    Illegal SapI site found at 6433
    Illegal SapI.rc site found at 10017


Design Notes

This plasmid was constructed by PCR SOEing.


Source

This plasmid was constructed by digesting pLV-TRE-Sox17-Ubc-Bla (BBa_K076001) with SfiI to excise Sox17, and inserting the PCR SOEd SfiI-Lbx1-T2A-GLRA1-P2A-D5R-SfiI.

References